Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, Guangzhou, 510632, Guangdong, People's Republic of China.
Cytotechnology. 2011 Aug;63(4):345-50. doi: 10.1007/s10616-011-9361-x. Epub 2011 May 17.
A novel, optical sensor was fixed in a new type of disposable bioreactor, Tubespin, for the on-line (real-time) monitoring of dissolved oxygen concentrations during cell culture. The cell density, viability and volumetric mass transfer coefficient were also determined to further characterize the bioreactors. The k(L)a value of the Tubespin at standard conditions was 24.3 h(-1), while that of a spinner flask was only 2.7 h(-1). The maximum cell density in the Tubespin bioreactor reached 6 × 10(6) cells mL(-1), which was two times higher than the cell density in a spinner flask. Furthermore, the dynamic dissolved oxygen level was maintained above 90% air-saturation in the Tubespin, while the value was only 1.9% in a spinner flask. These results demonstrate the competitive advantage of using the Tubespin system over spinner flasks for process optimization and scale-down studies of oxygen transfer and cell growth.
一种新型的光传感器被固定在一种新型的一次性生物反应器 Tubespin 中，用于在细胞培养过程中在线（实时）监测溶解氧浓度。还测定了细胞密度、活力和体积传质系数，以进一步表征生物反应器。在标准条件下，Tubespin 的 k(L)a 值为 24.3 h(-1)，而搅拌瓶仅为 2.7 h(-1)。Tubespin 生物反应器中的最大细胞密度达到 6×10(6)细胞 mL(-1)，是搅拌瓶中细胞密度的两倍。此外，Tubespin 中的动态溶解氧水平保持在高于 90%空气饱和度，而搅拌瓶中的值仅为 1.9%。这些结果表明，与搅拌瓶相比，使用 Tubespin 系统在优化工艺和氧传递及细胞生长的缩小研究方面具有竞争优势。
