Laboratory of Cellular Biotechnology, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, CH J2 506, Station 6, 1015 Lausanne, Switzerland.
Biotechnol Lett. 2011 May;33(5):897-902. doi: 10.1007/s10529-011-0527-6. Epub 2011 Jan 26.
Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 10(6) cells/ml, maximal cell densities of 16×10(6) and 6×10(6) cells/ml were reached in TubeSpins and spinner flasks, respectively. In addition the cell viability in these batch cultures remained above 90% for 10 days in TubeSpins but only for 4 days in spinner flasks. Inoculation at even higher cell densities reduced the duration of the lag phase. After inoculation at 2.5×10(6) cells/ml, the culture reached the maximum cell density within 3 days instead of 7 days as observed for inoculation with 10(6) cells/ml. Infection of Sf-9 cells in TubeSpins or spinner flasks with a recombinant baculovirus coding for green fluorescent protein (GFP) resulted in similar GFP-specific fluorescence levels. TubeSpins are thus an attractive option for the small-scale cultivation of Sf-9 cells in suspension and for baculovirus-mediated recombinant protein production.
在这里，我们展示了 TubeSpin 生物反应器 50（TubeSpins），这是一种用于悬浮 Sf-9 昆虫细胞的简单且一次性的培养系统。Sf-9 细胞在 TubeSpins 中的生长情况明显优于在搅拌瓶中。在以 10(6)个细胞/ml 接种后，TubeSpins 和搅拌瓶中分别达到了 16×10(6)和 6×10(6)个细胞/ml 的最大细胞密度。此外，在 TubeSpins 中，这些批次培养物中的细胞活力在 10 天内保持在 90%以上，但在搅拌瓶中仅为 4 天。以更高的细胞密度接种会缩短迟滞期的持续时间。以 2.5×10(6)个细胞/ml 接种后，培养物在 3 天内达到最大细胞密度，而不是以 10(6)个细胞/ml 接种时观察到的 7 天。在 TubeSpins 或搅拌瓶中用编码绿色荧光蛋白 (GFP)的重组杆状病毒感染 Sf-9 细胞，会产生相似的 GFP 特异性荧光水平。因此，TubeSpins 是悬浮培养 Sf-9 细胞和杆状病毒介导的重组蛋白生产的一种有吸引力的选择。
