Amirghofran Zahra, Javidnia Katayoun, Bahmani Masood, Azadmehr Abbas, Esmaeilbeig Maryam
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, IR Iran.
J Immunoassay Immunochem. 2011;32(3):157-69. doi: 10.1080/15321819.2011.552583.
In the present study, the immunomodulatory effects of Galium mite, a native herb used for the treatment of inflammation in Iranian traditional medicine, was investigated. The methanolic extract of the plant was prepared and examined for in vivo cell-mediated and humoral immunity against antigen in mice. Galium mite stimulated delayed type hypersensitivity at lower concentrations and inhibited the reaction at higher ones (p < 0.05). A dose-related decrease in primary and secondary antibody titer was observed in mice treated with the extract (p < 0.006). The extract at higher concentrations significantly reduced the proliferation of human-activated lymphocytes (p < 0.001). Cell cycle analysis on human lymphocytes treated with the extract showed an increase in the number of cells in sub-G1 region, indicating the ability of the extract to induce apoptosis in these cells. Induction of apoptosis was confirmed by DNA laddering on gel electrophoresis. In conclusion, G. mite has the ability to modulate cellular and humoral immune responses to the antigenic challenge and affect the rate of cell proliferation due to induction of apoptosis in the lymphocytes.
在本研究中，对伊朗传统医学中用于治疗炎症的本土草药小刺猪殃殃的免疫调节作用进行了研究。制备了该植物的甲醇提取物，并检测其对小鼠体内抗原介导的细胞免疫和体液免疫的影响。小刺猪殃殃在较低浓度下刺激迟发型超敏反应，在较高浓度下抑制该反应（p<0.05）。在用提取物处理的小鼠中观察到一级和二级抗体效价呈剂量相关下降（p<0.006）。较高浓度的提取物显著降低人活化淋巴细胞的增殖（p<0.001）。对用提取物处理的人淋巴细胞进行细胞周期分析，结果显示亚G1期细胞数量增加，表明提取物能够诱导这些细胞凋亡。通过凝胶电泳上的DNA梯带证实了凋亡的诱导。总之，小刺猪殃殃有能力调节对抗抗原攻击的细胞免疫和体液免疫反应，并由于诱导淋巴细胞凋亡而影响细胞增殖速率。
