Amirghofran Zahra, Bahmani Masoud, Azadmehr Abbas, Javidnia Katayoun
Department of Immunology, Shiraz University of Medical Science, Shiraz, Iran.
Med Sci Monit. 2007 Jun;13(6):BR145-50.
Several medicinal plants have shown anti-proliferative and apoptotic effects on human lymphocytes. In the present study the immunomodulatory properties of Stachys obtusicrena, a native plant to Iran, was examined and its possible effects on the induction of apoptosis were determined.
MATERIAL/METHODS: The in vivo effect of the methanolic extract of S. obtusicrena on delayed type hypersensitivity (DTH) and antibody production was investigated in mice immunized with the antigen. The proliferation of human lymphocytes stimulated with mitogen in the presence of the extract was determined by [3H]-thymidine incorporation assay. Mitogen-treated lymphocytes were exposed to the extract and analyzed for induction of apoptosis using flow cytometry and gel electrophoresis.
Data obtained from the DTH and antibody responses indicated a dose-related decrease in both parameters in the extract-treated mice compared with the untreated control group. In the in vitro study performed on the lymphocytes, the extract caused a dose-dependent decline in [3H]-thymidine uptake. The stimulation index of all cultures treated with different concentrations of the extract was less than 1. IL-2 levels in the culture supernatants of the activated lymphocytes showed a dose-related decrease as well. Results of flow cytometry indicated a dose-dependent accumulation of cells in the sub-G1 phase. The internucleosomal DNA fragmentation study showed typical DNA laddering in agarose gels.
The present study showed inhibitory effects of S. obtusicrena on both cellular and humoral immune responses and suggests that this effect may in part be due to the induction of apoptosis in proliferative lymphocytes.
几种药用植物已显示出对人淋巴细胞的抗增殖和凋亡作用。在本研究中，对伊朗本土植物钝齿水苏的免疫调节特性进行了检测，并确定了其对诱导凋亡的可能作用。
材料/方法：在用抗原免疫的小鼠中研究了钝齿水苏甲醇提取物对迟发型超敏反应（DTH）和抗体产生的体内作用。通过[3H]-胸腺嘧啶核苷掺入法测定在提取物存在下用丝裂原刺激的人淋巴细胞的增殖。将经丝裂原处理的淋巴细胞暴露于提取物中，并使用流式细胞术和凝胶电泳分析凋亡诱导情况。
从DTH和抗体反应获得的数据表明，与未处理的对照组相比，提取物处理的小鼠中这两个参数均呈剂量相关下降。在对淋巴细胞进行的体外研究中，提取物导致[3H]-胸腺嘧啶核苷摄取呈剂量依赖性下降。用不同浓度提取物处理的所有培养物的刺激指数均小于1。活化淋巴细胞培养上清液中的IL-2水平也呈剂量相关下降。流式细胞术结果表明，细胞在亚G1期呈剂量依赖性积累。核小体间DNA片段化研究显示琼脂糖凝胶中有典型的DNA梯带。
本研究表明钝齿水苏对细胞免疫和体液免疫反应均有抑制作用，并表明这种作用可能部分归因于增殖淋巴细胞中凋亡的诱导。
