Igaki N, Sakai M, Hata H, Oimomi M, Baba S, Kato H
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Clin Chem. 1990 Apr;36(4):631-4.
We investigated the effect of exogenously applied 3-deoxyglucosone, a major carbonyl intermediate, on the Maillard reaction. The fluorescence intensity of the product of the reaction of bovine serum albumin with 3-deoxyglucosone was higher than that with an equivalent amount of glucose. Similarly the rate of polymerization of lysozyme in the presence of 3-deoxyglucosone was also greater than with glucose, and collagen incubated with 3-deoxyglucosone was less digestible than collagen incubated with glucose. By contrast, aminoguanidine inhibited an increase in fluorescence of the Maillard compounds and the polymerization of protein, both of which were stimulated by 3-deoxyglucosone. These results suggest that 3-deoxyglucosone accelerates the advanced stage of the Maillard reaction and that aminoguanidine acts on 3-deoxyglucosone to inhibit its action in the advanced stage of the Maillard reaction.
我们研究了外源性应用的3-脱氧葡萄糖酮（一种主要的羰基中间体）对美拉德反应的影响。牛血清白蛋白与3-脱氧葡萄糖酮反应产物的荧光强度高于与等量葡萄糖反应产物的荧光强度。同样，在3-脱氧葡萄糖酮存在下溶菌酶的聚合速率也高于葡萄糖存在时，并且与3-脱氧葡萄糖酮一起孵育的胶原蛋白比与葡萄糖一起孵育的胶原蛋白更难消化。相比之下，氨基胍抑制了美拉德化合物荧光的增加以及蛋白质的聚合，而这两者均受到3-脱氧葡萄糖酮的刺激。这些结果表明，3-脱氧葡萄糖酮加速了美拉德反应的晚期阶段，并且氨基胍作用于3-脱氧葡萄糖酮以抑制其在美拉德反应晚期阶段的作用。
