School of Paediatrics and Reproductive Health, Robinson Institute, University of Adelaide, North Adelaide, South Australia, Australia.
Fertil Steril. 2011 Jul;96(1):47-52.e2. doi: 10.1016/j.fertnstert.2011.04.033. Epub 2011 May 14.
To identify the cumulus cell gene expression associated with oocyte developmental competence, specifically live birth, after single ET (SET) assisted reproductive technology.
Retrospective gene expression analysis in human cumulus cells from oocytes that established a pregnancy resulting in live birth versus no pregnancy after SET.
Independent IVF clinic and research institute.
PATIENT(S): Women undergoing IVF/intracytoplasmic sperm injection with SET.
INTERVENTION(S): Quantitative reverse-transcriptase-polymerase chain reaction analysis was performed on cumulus masses collected before insemination. Oocytes and embryos were cultured and transferred independently in 38 patients undergoing elective SET. Paired cumulus samples from oocytes that developed into high- versus low-grade embryos also were compared.
MAIN OUTCOME MEASURE(S): Gene expression profiles of metabolic (ALDOA, LDHA, PFKP, PKM2), signaling (AHR, GREM1, PTGS2, STS), extracellular matrix (HAS2, PTX3, TNFAIP6, VCAN), and loading control GAPDH in individual cumulus masses.
RESULT(S): VCAN and PTGS2 mRNA expression was significantly higher in cumulus cells from oocytes yielding a pregnancy resulting in a live birth, while PTX3 mRNA expression trended toward higher expression in pregnant samples. Cumulus cell levels of VCAN, GREM1, and PFKP correlated with birth weight in patients at 38 weeks of gestation. No genes correlated with clinical embryo morphology scores.
CONCLUSION(S): Cumulus cell VCAN, PTGS2, GREM1, and PFKP expression may identify oocytes with high developmental potential, leading to enhanced implantation rates and greater developmental capacity throughout gestation.
鉴定与卵母细胞发育能力相关的卵丘细胞基因表达，特别是在单胚胎移植（SET）辅助生殖技术后获得活产。
人类卵丘细胞中基因表达的回顾性分析，这些卵丘细胞来自 SET 后成功妊娠并分娩的卵母细胞与 SET 后未妊娠的卵母细胞。
独立的体外受精（IVF）诊所和研究所。
接受 SET 的 IVF/胞浆内精子注射的女性。
在授精前收集卵丘细胞进行定量逆转录-聚合酶链反应分析。38 名接受选择性 SET 的患者中，将卵母细胞和胚胎分别进行培养和移植。还比较了发育为高低等级胚胎的卵母细胞的配对卵丘样本。
单个卵丘样本中代谢（ALDOA、LDHA、PFKP、PKM2）、信号（AHR、GREM1、PTGS2、STS）、细胞外基质（HAS2、PTX3、TNFAIP6、VCAN）和加载控制 GAPDH 的基因表达谱。
在导致妊娠并分娩的卵母细胞的卵丘细胞中，VCAN 和 PTGS2 mRNA 表达显著升高，而 PTX3 mRNA 表达在妊娠样本中呈上升趋势。在 38 周妊娠的患者中，卵丘细胞中 VCAN、GREM1 和 PFKP 的水平与出生体重相关。没有基因与临床胚胎形态评分相关。
卵丘细胞 VCAN、PTGS2、GREM1 和 PFKP 的表达可能鉴定出具有高发育潜能的卵母细胞，从而提高着床率，并在整个妊娠过程中提高发育能力。
