麻醉对体内乙酰胆碱传递的影响；放射酶法与高效液相色谱法测定的比较。

Anaesthesia effects on in vivo acetylcholine transmission; comparisons of radioenzymatic and HPLC assays.

作者信息

Bertorelli R, Hallström A, Hurd Y L, Karlsson A, Consolo S, Ungerstedt U

机构信息

Mario Negri Instituto Di Ricerche Farmacologiche, Milano, Italy.

出版信息

Eur J Pharmacol. 1990 Jan 3;175(1):79-83. doi: 10.1016/0014-2999(90)90155-y.

DOI:10.1016/0014-2999(90)90155-y

PMID:2157598

Abstract

The effect of general anaesthesia on extracellular levels of acetylcholine (ACh) in the caudate-putamen of freely moving rats was studied by microdialysis. ACh concentrations were determined in the same perfusate samples by radioenzymatic and HPLC/electrochemical procedures in order to compare the assays. The concentration of ACh in perfusate samples was estimated to be 0.30 microM in conscious unrestrained rats. However, when these rats were administered chloral hydrate (400 mg/kg i.p.), the level of ACh was decreased immediately by 50%, attaining a value of 0.06 microM within 20-40 min following the injection. Upon recovery of the righting reflex, ACh levels were once again re-elevated. The levels of choline (Ch), the precursor of ACh, were unaffected by anaesthesia. It was apparent that the level of consciousness (i.e. awake vs. anaesthetized) is an important factor determining ACh overflow. Radioenzymatic and HPLC assays proved to give identical results for the analysis of ACh and Ch.

摘要

通过微透析研究了全身麻醉对自由活动大鼠尾状核-壳核中乙酰胆碱（ACh）细胞外水平的影响。为了比较分析方法，采用放射酶法和高效液相色谱/电化学方法测定相同灌流液样品中的ACh浓度。清醒不受约束的大鼠灌流液样品中ACh的浓度估计为0.30微摩尔。然而，当给这些大鼠腹腔注射水合氯醛（400毫克/千克）时，ACh水平立即下降50%，在注射后20-40分钟内达到0.06微摩尔的值。恢复翻正反射后，ACh水平再次升高。ACh的前体胆碱（Ch）水平不受麻醉影响。显然，意识水平（即清醒与麻醉）是决定ACh溢出的一个重要因素。放射酶法和高效液相色谱法对ACh和Ch的分析结果相同。