Ajima A, Kato T
Department of Life Chemistry, Graduate School at Nagatsuta, Tokyo Institute of Technology, Yokohama, Japan.
Neurosci Lett. 1987 Oct 16;81(1-2):129-32. doi: 10.1016/0304-3940(87)90352-1.
A dialysis cannula was implanted into rat striatum while the animals were anesthetized, and after at least one day following the surgery the area was perfused with Ringer solution under the unrestrained and unanesthetized conditions. Concentration of acetylcholine (ACh) in the perfusate was determined by high-performance liquid chromatography (HPLC)-electrochemical detection (ECD) with the enzyme-column on which acetylcholine esterase and choline oxidase were immobilized. ACh in the dialysate was only detectable when the Ringer solution containing eserine, an inhibitor of acetylcholinesterase, was perfused. ACh peak on HPLC-ECD could be detected at least for 4 h under these conditions. The level of ACh increased 2-3 fold with the perfusion of 1 mM atropine sulfate, a blocker of ACh receptor. These data indicate that brain dialysis in the presence of eserine is useful for study on the neurochemical activity of ACh neurons in the brain.
在大鼠麻醉状态下将透析套管植入纹状体，术后至少一天后，在无束缚、未麻醉的条件下用林格液对该区域进行灌流。灌流液中乙酰胆碱（ACh）的浓度通过高效液相色谱（HPLC）-电化学检测（ECD），使用固定有乙酰胆碱酯酶和胆碱氧化酶的酶柱进行测定。仅当灌注含有乙酰胆碱酯酶抑制剂毒扁豆碱的林格液时，透析液中的ACh才可检测到。在这些条件下，HPLC-ECD上的ACh峰至少可检测4小时。随着1 mM硫酸阿托品（一种ACh受体阻滞剂）的灌注，ACh水平增加2至3倍。这些数据表明，在毒扁豆碱存在下进行脑透析有助于研究脑中ACh神经元的神经化学活性。
