School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2011 Jul;102(14):7147-53. doi: 10.1016/j.biortech.2011.04.028. Epub 2011 Apr 14.
The inhibitory effect of organic carbon on CO(2) fixation (CF) by the non-photosynthetic microbial community (NPMC) and its mechanism were studied. The results showed that different concentrations of glucose inhibited CF to some extent. However, when these microorganisms pre-cultured with glucose were re-cultured without organic carbon, their CF efficiency differed significantly from the control based on the glucose concentration in the pre-culture. ATP as bioenergy and NADH as reductant had no obvious inhibitory effect on CF; conversely, they improved CF efficiency to some extent, especially when both were present simultaneously. These results implied that not all organic materials inhibited CF by NPMC, and only those that acted as good carbon sources, such as glucose, inhibited CF. Moreover, some metabolites generated during the catabolism of glucose by heterotrophic metabolism of NPMC might inhibit CF, while other cumulated materials present in the cell interior, such as ATP and NADH, might improve CF.
研究了有机碳对非光合微生物群落（NPMC）固定 CO2（CF）的抑制作用及其机制。结果表明，不同浓度的葡萄糖对 CF 有一定的抑制作用。然而，当这些微生物在预培养中用葡萄糖培养后，在没有有机碳的情况下再培养，它们的 CF 效率与预培养中葡萄糖浓度的对照相比有显著差异。生物能量的 ATP 和还原剂 NADH 对 CF 没有明显的抑制作用；相反，它们在一定程度上提高了 CF 效率，特别是当两者同时存在时。这些结果表明，并非所有的有机物质都能抑制 NPMC 的 CF，只有那些能作为良好碳源的物质，如葡萄糖，才能抑制 CF。此外，NPMC 异养代谢过程中葡萄糖分解产生的一些代谢产物可能会抑制 CF，而细胞内其他累积的物质，如 ATP 和 NADH，则可能会提高 CF。
