Hu Jiajun, Wang Lei, Zhang Shiping, Xi Xuefei, Le Yiquan, Fu Xiaohua, Tsang Yiufai, Gao Mintian
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.
Appl Biochem Biotechnol. 2015 Jul;176(5):1459-71. doi: 10.1007/s12010-015-1657-4. Epub 2015 May 7.
Five autotrophic strains isolated from non-photosynthetic microbial communities (NPMCs), which were screened from oceans with high CO2 fixing capability, were identified as Ochrobactrum sp. WH-2, Stenotrophomonas sp. WH-11, Ochrobactrum sp. WH-13, Castellaniella sp. WH-14, and Sinomicrobium oceani WH-15. The CO2 fixation pathways of all these strains were Calvin-Benson-Bassham pathway. These strains could metabolize multifarious organic compounds, which allowed switching them to autotrophic culture after enrichment in heterotrophic culture. The central composite response surface method indicated that these strains possessed many interactive effects, which increased the CO2 fixing efficiency of a combined community composed of these strains by 56 %, when compared with that of the single strain. Furthermore, another combined community composed of these autotrophic strains and NPMC had richer interactive relationships, with CO2 fixing efficiency being 894 % higher than that of the single strain and 148 % higher than the theoretical sum of the CO2 fixing efficiency of each of its microbial components. The interaction between strictly heterotrophic bacteria in NPMC and isolated autotrophic strains played a crucial role in improving the CO2 fixing efficiency, which not only eliminated self-restraint of organic compounds generated during the growth of autotrophic bacteria but also promoted its autotrophic pathway.
从具有高二氧化碳固定能力的海洋中筛选出的非光合微生物群落（NPMCs）中分离出的五株自养菌株，被鉴定为苍白杆菌属WH-2、嗜麦芽窄食单胞菌属WH-11、苍白杆菌属WH-13、卡氏菌属WH-14和海洋中华微菌WH-15。所有这些菌株的二氧化碳固定途径均为卡尔文-本森-巴斯姆途径。这些菌株能够代谢多种有机化合物，这使得它们在异养培养富集后可转换为自养培养。中心复合响应面法表明，这些菌株具有许多交互作用，与单一菌株相比，由这些菌株组成的组合群落的二氧化碳固定效率提高了56%。此外，由这些自养菌株和NPMC组成的另一个组合群落具有更丰富的交互关系，其二氧化碳固定效率比单一菌株高894%，比其每个微生物组分的二氧化碳固定效率理论总和高148%。NPMC中严格异养细菌与分离出的自养菌株之间的相互作用在提高二氧化碳固定效率方面起着关键作用，这不仅消除了自养细菌生长过程中产生的有机化合物的自我抑制，还促进了其自养途径。
