Faculdade de Engenharia Elétrica e Computação, Universidade de Campinas, Campinas, SP, Brazil.
Nanotechnology. 2011 Jul 1;22(26):265103. doi: 10.1088/0957-4484/22/26/265103. Epub 2011 May 17.
Our data demonstrate that multi-walled carbon nanotubes (MWCNTs) are internalized by macrophages, subsequently activating them to produce interleukin (IL)-12 (IL-12). This cytokine induced the proliferative response of T lymphocytes to a nonspecific mitogen and to ovalbumin (OVA). This increase in the proliferative response was accompanied by an increase in the expression of pro-inflammatory cytokines, such as interferon-gamma (IFNγ), tumor necrosis factor-alpha (TNFα) and IL-6, in mice inoculated with MWCNTs, whether or not they had been immunized with OVA. A decrease in the expression of transforming growth factor-beta (TGFβ) was observed in the mice treated with MWCNTs, whereas the suppression of the expression of both TGFβ and IL-10 was observed in mice that had been both treated and immunized. The activation of the T lymphocyte response by the pro-inflammatory cytokines leads to an increase in antibody production to OVA, suggesting the important immunostimulatory effect of carbon nanotubes.
我们的数据表明，多壁碳纳米管（MWCNTs）被巨噬细胞内化，随后激活它们产生白细胞介素（IL）-12（IL-12）。这种细胞因子诱导 T 淋巴细胞对非特异性有丝分裂原和卵清蛋白（OVA）的增殖反应。这种增殖反应的增加伴随着促炎细胞因子的表达增加，如干扰素-γ（IFNγ）、肿瘤坏死因子-α（TNFα）和 IL-6，在接种 MWCNTs 的小鼠中观察到，无论它们是否用 OVA 免疫。在接受 MWCNTs 治疗的小鼠中观察到转化生长因子-β（TGFβ）的表达下降，而在既接受治疗又接受免疫的小鼠中观察到 TGFβ 和 IL-10 的表达均受到抑制。促炎细胞因子激活 T 淋巴细胞反应导致对 OVA 的抗体产生增加，这表明碳纳米管具有重要的免疫刺激作用。
