Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Microbes Environ. 2010;25(1):36-40. doi: 10.1264/jsme2.me09177.
We established artificial consortia of bacteria inoculated from soil and a green alga, Chlorella vulgaris NIES-227. The bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. The community of bacteria formed in the consortia included those phylogenetically closely related to the genera Phenylobacterium, Brevundimonas, Phyllobacterium, Afipia, Sphingomonas, Sandaracinobacter, Ramlibacter, Ralstonia, Cellvibrio, and so on. The community also included bacteria belonging to unknown genera within the phyla Bacteroidetes, Verrucomicrobia, and Acidobacteria. It was indicated that Ralstonia sp. and Cellvibrio sp. were attached to the algal cells and Phenylobacterium sp. Ramlibacter sp., Methylophilus sp., and Rhizobiaceae bacterium were not. It was suggested that bacteria belonging to the phylum Bacteroidetes and the class Alphaproteobacteria could be hemi-selectively enriched by the alga.
我们从土壤和绿藻小球藻 NIES-227 中建立了人工细菌共生体。将细菌和藻类混合在试管中或在透析培养容器中进行分区培养，然后检查培养后的细菌组成。在共生体中形成的细菌群落包括与 Phenylobacterium、Brevundimonas、Phyllobacterium、Afipia、Sphingomonas、Sandaracinobacter、Ramlibacter、Ralstonia、Cellvibrio 等属密切相关的细菌。该群落还包括属于厚壁菌门、疣微菌门和酸杆菌门未知属的细菌。结果表明，Ralstonia sp. 和 Cellvibrio sp. 附着在藻类细胞上，而 Phenylobacterium sp. Ramlibacter sp.、Methylophilus sp. 和 Rhizobiaceae 细菌则没有。这表明属于拟杆菌门和α变形菌纲的细菌可以被藻类半选择性地富集。
