Permeen A M, Sam C K, Pathmanathan R, Prasad U, Wolf H
Nasopharyngeal Carcinoma Research Laboratory, University of Malaya, Kuala Lumpur, West Malaysia.
J Virol Methods. 1990 Mar;27(3):261-7. doi: 10.1016/0166-0934(90)90094-v.
The presence of Epstein Barr virus (EBV) DNA in biopsies from the post-nasal space (PNS) of patients suspected of nasopharyngeal carcinoma (NPC) was detected by in situ cytohybridization with an EBV DNA probe labelled with the novel labelling compound digoxigenin. The digoxigenin probe was hybridised to cryostat sections of NPC biopsies and subsequently detected by an enzyme immunoassay procedure. It was found that in situ cytohybridization using the digoxigenin probe was much more rapid and sensitive (96 h compared to five weeks) than the current method of using 3H-labelled probe. Using the digoxigenin EBV probe, it was found that in all the eighteen NPC biopsies tested, EBV DNA was detected in malignant epithelial cells and infiltrating lymphocytes. EBV DNA was also detected in some normal epithelial cells in these NPC biopsies. EBV DNA was not detected in epithelial cells of non-malignant biopsies.
采用用新型标记化合物地高辛标记的EBV DNA探针，通过原位细胞杂交法检测疑似鼻咽癌（NPC）患者后鼻孔区（PNS）活检组织中EB病毒（EBV）DNA的存在情况。将地高辛探针与NPC活检组织的冰冻切片进行杂交，随后通过酶免疫测定法进行检测。结果发现，与目前使用3H标记探针的方法相比，使用地高辛探针进行原位细胞杂交要快速得多且灵敏得多（分别为96小时和五周）。使用地高辛EBV探针发现，在所有检测的18例NPC活检组织中，在恶性上皮细胞和浸润淋巴细胞中均检测到EBV DNA。在这些NPC活检组织的一些正常上皮细胞中也检测到了EBV DNA。在非恶性活检组织的上皮细胞中未检测到EBV DNA。
