不稳定的关联：转座子在植物基因工程中的应用

Unstable liaisons: the use of transposons in plant genetic engineering.

作者信息

Spena A

机构信息

MPI für Züchtungsforschung, Köln, FRG.

出版信息

Trends Genet. 1990 Mar;6(3):76-7. doi: 10.1016/0168-9525(90)90097-p.

DOI:10.1016/0168-9525(90)90097-p

PMID:2158163

Abstract

Transposable elements can be used to activate gene expression in clonal populations of cells and consequently to generate genetic mosaics. Plant mosaics, having organs composed of intermixed tissues with wild-type and mutant appearance, can be used to evaluate whether the action of a morphogenetic gene is cell-autonomous. Transposon-split gene constructions could also be used to raise plants transgenic for lethal genes and, in more general terms, they could be an ancillary tool in several types of biological experiment.

摘要

转座元件可用于激活细胞克隆群体中的基因表达，从而产生遗传镶嵌体。植物镶嵌体的器官由具有野生型和突变外观的混合组织组成，可用于评估形态发生基因的作用是否是细胞自主的。转座子分裂基因构建体也可用于培育携带致死基因的转基因植物，更一般地说，它们可以作为几种生物学实验中的辅助工具。