Krishnaswamy Lakshminarasimhan, Peterson Thomas
Department of Biology, Singapore University of Technology and Design (SUTD), Singapore, Singapore.
Methods Mol Biol. 2013;1057:43-58. doi: 10.1007/978-1-62703-568-2_4.
Marker genes have played a critical role in the discovery of plant transposable elements, our understanding of transposon biology, and the utility of transposable elements as tools in functional genomics. Marker traits in model plants have been useful to detect transposable elements and to study the dynamics of transposition. Transposon-induced changes in the sequence of marker genes and consequently their expression have contributed to our understanding of molecular mechanisms of transposition and associated genome rearrangements. Further, marker genes that have been cloned and are compatible in heterologous systems have found versatile utility in the design of DNA constructs that have enabled us to understand the finer details of transposition mechanisms, and also allowed the use of transposon-based tools for functional genomics. This chapter traces the role of marker traits and marker genes (endogenous and transgenic) in various plant systems, and their contributions to the advancement of transposon biology over the past several decades.
标记基因在植物转座子的发现、我们对转座子生物学的理解以及转座子作为功能基因组学工具的应用方面发挥了关键作用。模式植物中的标记性状有助于检测转座子并研究转座动态。转座子诱导的标记基因序列变化及其表达变化有助于我们理解转座的分子机制以及相关的基因组重排。此外，已克隆且在异源系统中兼容的标记基因在DNA构建体设计中具有广泛用途，这些构建体使我们能够了解转座机制的更细微细节，还允许使用基于转座子的工具进行功能基因组学研究。本章追溯了标记性状和标记基因（内源和转基因）在各种植物系统中的作用，以及它们在过去几十年对转座子生物学发展的贡献。
