通过苯酚与硫酸或苯酚与高氯酸比色微量法测定戊二醛。

Colorimetric micromethods for glutaraldehyde determination by means of phenol and sulfuric acid or phenol and perchloric acid.

作者信息

Boratyński J, Zal T

机构信息

Department of Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.

出版信息

Anal Biochem. 1990 Feb 1;184(2):259-62. doi: 10.1016/0003-2697(90)90677-2.

DOI:10.1016/0003-2697(90)90677-2

PMID:2158247

Abstract

Aqueous micromolar solutions of glutaraldehyde produce a yellow color when treated with 20% phenol in ethanol followed by concentrated sulfuric acid or with phenol in 70% perchloric acid. The absorbance at 482 or 479 nm, respectively, is linearly related to the glutaraldehyde concentration. Of the two methods developed, the sulfuric acid-phenol assay gives a higher sensitivity and the perchloric acid-phenol assay allows the determination of glutaraldehyde in the presence of sugars and proteins.

摘要

当用乙醇中的20%苯酚，接着用浓硫酸处理，或用70%高氯酸中的苯酚处理时，戊二醛的微摩尔水溶液会产生黄色。在482或479 nm处的吸光度分别与戊二醛浓度呈线性相关。在所开发的两种方法中，硫酸-苯酚法灵敏度更高，高氯酸-苯酚法可在有糖和蛋白质存在的情况下测定戊二醛。