Chayavanich Kasitnun, Sapyen Wannida, Imyim Apichat
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Nanotec-CU Center of Excellence on Food and Agriculture, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Mar 5;308:123761. doi: 10.1016/j.saa.2023.123761. Epub 2023 Dec 12.
A user-friendly platform based on foam sheets and silica solid support was developed for colorimetric sensing of dextran via phenol-sulfuric acid assay. The chemical reagents, sulfuric acid and phenol were separately preserved by silica gel. The brown color product of the phenol-sulfuric acid reaction occurred on the silica solid support when sample solutions were added. The color intensity of brown products was easily obtained by a smartphone and color processing software. Subsequently, a broad concentration range of dextran could be determined up to 10,000 mg/L dextran, with a detection limit of 360 mg/L. Furthermore, a precision study, including inter-day and intra-day studies, presented a satisfactory performance for dextran detection. The developed platform was successfully applied for the sugar industry's dextran determination of syrup samples.
基于泡沫片和硅胶固体支持物开发了一种用户友好型平台,用于通过苯酚-硫酸法比色传感葡聚糖。化学试剂硫酸和苯酚分别由硅胶保存。加入样品溶液后,苯酚-硫酸反应的棕色产物在硅胶固体支持物上产生。棕色产物的颜色强度可通过智能手机和颜色处理软件轻松获得。随后,可测定高达10,000 mg/L葡聚糖的宽浓度范围,检测限为360 mg/L。此外,包括日间和日内研究的精密度研究表明,该平台在葡聚糖检测方面具有令人满意的性能。所开发的平台成功应用于制糖工业糖浆样品中葡聚糖的测定。