Methods for enzymatic and colorimetric determinations of D-erythrulose (D-tetrulose).
作者信息
Morii K, Hosomi S, Terada T, Mizoguchi T
出版信息
Anal Biochem. 1985 Nov 15;151(1):188-91. doi: 10.1016/0003-2697(85)90070-3.
PMID:4091279
Abstract
The specific determinations of D-erythrulose by enzymatic assay or colorimetric method, which permit the quantitative determination of between 20 and 400 nmol of the sugar, are described. Enzymatic determination of D-erythrulose made use of the D-erythrulose reductase purified from beef or chicken liver, which catalyzes specifically the reduction of D-erythrulose with concomitant conversion of NADH to NAD+. The colorimetric microdetermination of erythrulose could be carried out by utilizing the phenol-sulfuric acid reaction under low temperature. These methods are simple, rapid, and sensitive, and give reproducible results.
摘要
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