Mengeling W L, Matthews P J
USDA, National Animal Disease Center, Ames, IA 50010.
Am J Vet Res. 1990 Apr;51(4):632-5.
The ability of pigs to respond immunologically to ingestion of bovine parvovirus (BPV) was tested by feeding 4 cesarean-derived, colostrum-deprived (CDCD) pigs a live virus-contaminated, liquid diet for the first 4 weeks of life. Virus-neutralizing (VN) antibodies were detected in the serum of 2 of the 4 pigs when they were 4 weeks old. Antibody titer remained at about the same level for several weeks, then decreased during the remainder of the 29-week interval of testing. The relative reactivity of these sera based on results of indirect immunofluorescence paralleled the corresponding VN titer. Neither of the other 2 pigs exposed to BPV had any appreciable immune response. The potential for passive acquisition of antibody from the diet was tested by feeding 4 other CDCD pigs bovine colostrum containing antibodies to BPV and bovine viral diarrhea virus (BVDV) for the first 2 days of life. All had serum VN antibodies for both viruses when they were tested at 2 days of age. The decay rate of the heterologous, passively acquired antibody was approximately linear; however, antibody half-life was relatively short, about 3.5 days, and titers were no longer detectable when pigs were 4 weeks (BPV) and 6 weeks (BVDV) old. An additional 4 CDCD pigs fed a liquid diet without virus or antibody remained free of any appreciable serum reactivity for either BPV or BVDV. Results supported the hypothesis that antibodies for BPV previously detected in the serum of pigs and people may reflect ingestion of virus-contaminated bovine milk or milk products.
通过在出生后的前4周给4头剖腹产、未摄入初乳的（CDCD）仔猪喂食受活病毒污染的流质饮食，测试了仔猪对摄入牛细小病毒（BPV）的免疫反应能力。4头仔猪中有2头在4周龄时血清中检测到病毒中和（VN）抗体。抗体滴度在几周内保持在大致相同的水平，然后在29周测试期的剩余时间内下降。基于间接免疫荧光结果，这些血清的相对反应性与相应的VN滴度平行。另外2头接触BPV的仔猪均未产生任何明显的免疫反应。通过在出生后的前两天给另外4头CDCD仔猪喂食含有BPV和牛病毒性腹泻病毒（BVDV）抗体的牛初乳，测试了从饮食中被动获得抗体的可能性。在2日龄时对它们进行检测时，所有仔猪血清中均含有这两种病毒的VN抗体。异源被动获得抗体的衰减率大致呈线性；然而，抗体半衰期相对较短，约为3.5天，当仔猪4周龄（BPV）和6周龄（BVDV）时，滴度不再可检测到。另外4头喂食不含病毒或抗体的流质饮食的CDCD仔猪对BPV或BVDV均未产生任何明显的血清反应。结果支持了以下假设：先前在猪和人的血清中检测到的BPV抗体可能反映了摄入受病毒污染的牛奶或奶制品。
