Bolin S R, Ridpath J F
National Animal Disease Center, US Department of Agriculture, Ames, LA 50010.
Am J Vet Res. 1989 Jun;50(6):817-21.
Serum was obtained at weekly intervals after vaccination of 6 healthy calves with either of 2 commercially available monovalent modified-live bovine viral diarrhea (BVD) virus vaccines. Detectable neutralizing antibodies to each of 10 cytopathic and 10 noncytopathic isolates of BVD virus were produced by 1 or more of the calves by 14 days after vaccination, but no calf produced detectable neutralizing antibodies to all 20 BVD viruses. At that time, precipitating antibodies against viral-induced polypeptides of approximately 115,000; 80,000; 56,000; 48,000; 39,000; and 25,000 daltons were detected in sera from some calves. Also at that time, specificity of the antibodies for polypeptides of certain viruses was detected. At 21 days after vaccination, each calf produced neutralizing antibodies to all 20 BVD viruses. At that time, precipitating antibodies to each of the aforementioned viral induced polypeptides were detected in serum from each calf. Precipitating antibodies to viral induced polypeptides of 61,000 and 37,000 daltons were detected in samples of sera obtained from some calves at 42 days after vaccination.
用两种市售单价改良活牛病毒性腹泻（BVD）病毒疫苗之一对6头健康小牛进行疫苗接种后，每周采集血清。接种后14天，1头或多头小牛产生了针对10种细胞病变型和10种非细胞病变型BVD病毒分离株中每种病毒的可检测中和抗体，但没有一头小牛产生针对所有20种BVD病毒的可检测中和抗体。此时，在一些小牛的血清中检测到针对病毒诱导的约115,000；80,000；56,000；48,000；39,000和25,000道尔顿多肽的沉淀抗体。同样在此时，检测到抗体对某些病毒多肽的特异性。接种后21天，每头小牛都产生了针对所有20种BVD病毒的中和抗体。此时，在每头小牛的血清中检测到针对上述每种病毒诱导多肽的沉淀抗体。在接种后42天从一些小牛获得的血清样本中检测到针对61,000和37,000道尔顿病毒诱导多肽的沉淀抗体。
