Kimura H, Yonemura Y, Miyazaki I
KANAZAWA UNIV,SCH MED,DEPT SURG 2,KANAZAWA,ISHIKAWA 920,JAPAN.
Int J Oncol. 1992 Aug;1(3):265-9. doi: 10.3892/ijo.1.3.265.
By setting the cut-off line at the level as used in a negative control study without primary antibody in the same sample, the Ki-67 labeling rate was calculated by the Ki67-DNA dual fluorescence analysis. The mean Ki-67 labeling rate of 28 advanced gastric cancer was 45.1% (13.9-76.3%). The Ki-67 labeling rates were significantly higher for larger size tumor (p<0.05), peritoneal metastasis and advanced stage (p<0.01). This is the first report that cycling cells are calculated by setting the cut-off line for solid tumors. Use of this flow cytometric procedure substantially facilitates the quantification of proliferating cells in gastric cancer.
通过将截止线设定在与同一样本中无一抗的阴性对照研究中所使用的水平，采用Ki67-DNA双荧光分析计算Ki-67标记率。28例进展期胃癌的平均Ki-67标记率为45.1%（13.9 - 76.3%）。肿瘤体积较大（p<0.05）、有腹膜转移及处于晚期（p<0.01）的患者，其Ki-67标记率显著更高。这是首篇通过为实体瘤设定截止线来计算循环细胞的报告。这种流式细胞术程序的使用极大地促进了胃癌中增殖细胞的定量分析。
