Department of Parasitology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
Parasitol Res. 2011 Dec;109(6):1637-46. doi: 10.1007/s00436-011-2435-z. Epub 2011 May 17.
Toxoplasma gondii is an obligate intracellular protozoan parasite, which can invade and multiply within the macrophages of humans and most warm-blooded animals. Macrophages are important effector cells for the control and killing of intracellular T. gondii, and they may also serve as long-term host cells for the replication and survival of the parasite. In the present study, we explored the proteomic profile of macrophages of the specific pathogen-free Kunming mice at 24 h after infection with tachyzoites of the virulent T. gondii RH strain using two-dimensional gel electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight (TOF)/TOF tandem mass spectrometry. Totally, 60 differentially expressed protein spots were identified. Among them, 52 spots corresponded to 38 proteins matching to proteins of the mouse, including actin, enolase, calumenin, vimentin, plastin 2, annexin A1, cathepsin S, arginase-1, arachidonate 12-lipoxygenase, and aminoacylase-1. Functional prediction using Gene Ontology database showed that these proteins were mainly involved in metabolism, structure, protein fate, and immune responses. The findings provided an insight into the interactive relationship between T. gondii and the host macrophages, and will shed new lights on the understanding of molecular mechanisms of T. gondii pathogenesis.
刚地弓形虫是一种专性细胞内寄生的原虫，能够侵入并在人类和大多数温血动物的巨噬细胞内繁殖。巨噬细胞是控制和杀死细胞内弓形虫的重要效应细胞，也可能作为寄生虫复制和存活的长期宿主细胞。在本研究中，我们使用二维凝胶电泳结合基质辅助激光解吸电离飞行时间（TOF）/TOF 串联质谱技术，研究了感染强毒 RH 株速殖子后无特定病原体昆明小鼠巨噬细胞的蛋白质组图谱。共鉴定出 60 个差异表达蛋白斑点。其中，52 个斑点与 38 种与小鼠蛋白质相对应的蛋白质相对应，包括肌动蛋白、烯醇酶、钙网蛋白、波形蛋白、肌动蛋白、膜联蛋白 A1、组织蛋白酶 S、精氨酸酶-1、花生四烯酸 12-脂氧合酶和氨基酰酶-1。使用基因本体数据库进行功能预测表明，这些蛋白质主要参与代谢、结构、蛋白质命运和免疫反应。研究结果深入了解了弓形虫与宿主巨噬细胞之间的相互关系，将为理解弓形虫发病机制的分子机制提供新的思路。
