转座子Tn916从枯草芽孢杆菌向嗜热栖热菌的转移。

Transfer of transposon Tn916 from Bacillus subtilis to Thermus aquaticus.

作者信息

Sen S, Oriel P

机构信息

Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.

出版信息

FEMS Microbiol Lett. 1990 Jan 15;55(1-2):131-4. doi: 10.1016/0378-1097(90)90181-o.

DOI:10.1016/0378-1097(90)90181-o

PMID:2158473

Abstract

Broad host range conjugating transposon Tn916 has been introduced into the extreme thermophile Thermus by transposon transformation and transposition into the Bacillus subtilis chromosome followed by broth mating with Thermus aquaticus ATCC27634. Tetracycline resistant Thermus transconjugants were obtained at a frequency of 1.4 X 10(-7) per donor and 1.2 X 10(-7) per recipient. Transposon transfer from Thermus to Bacillus subtilis was also demonstrated in similar broth matings. Transfer characteristics were consistent with the conjugation mechanism described for Tn916 in mesophiles.

摘要

广宿主范围接合转座子Tn916已通过转座子转化被导入嗜热栖热放线菌，它先转座到枯草芽孢杆菌染色体上，然后与嗜热水栖热放线菌ATCC27634进行肉汤交配。获得了对四环素耐药的嗜热栖热放线菌接合子，其频率为每个供体1.4×10⁻⁷，每个受体1.2×10⁻⁷。在类似的肉汤交配中也证明了转座子从嗜热栖热放线菌转移到枯草芽孢杆菌。转移特性与在嗜温菌中描述的Tn916的接合机制一致。