温针艾灸疗法可能减轻阿司匹林诱导的大鼠胃黏膜损伤

[Warm-needle moxibustion therapy may reduce aspirin-induced gastric mucosal injury in rats].

作者信息

Sun Shi-lin, Sun Zhong-ren, Liu Min, Li Chao-ming, He Wei, Gao Ting, Liu Ming

机构信息

Heilongjiang University of Chinese Medicine, Harbin 150040, China.

出版信息

Zhen Ci Yan Jiu. 2011 Feb;36(1):28-31.

PMID:21585055

Abstract

OBJECTIVE

To observe the effect of warm-needle moxibustion on the gastric mucosal injury caused by anti-rheumatic dose of Aspirin.

METHODS

Forty SD rats were equally randomized into blank group, model group, warm needling group and Omeprazole (for relieving peptic ulcer) group, with 10 rats in each. The rats were forced to undergo 12 hours' fasting and 4 hours' water-deprivation before the experiments. Normal saline (10 mL/kg) was given to the rats in the blank group. Gastric mucosa lesion model was established by intragastric infusion of aspirin (420 mg/kg). Warm needling was applied to "Zhong-wan" (CV 12), "Xiawan" (CV 10), "Zusanli" (ST 36) and "Neiguan" (PC 6) for 30 min, once a day for 7 days. The rats of the Omeprazole group were fed with Omeprazole( 10 mg/100 g body weight) once a day for 7 days. According to Guth's method, the ulcer index (UI) of the gastric mucosa was calculated following killing the rats. The activity of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) in the serum and gastric mucosa were detected with hydroxylamine method and thiobarbituric acid method, respectively.

RESULTS

Compared with the blank group, the gastric UI, MDA levels of both serum and gastric mucosa in the model group were increased obviously (P<0. 01), while serum and gastric mucosal SOD activity in the model group were decreased considerably (P<0. 01). In comparison with the model group, the gastric UI, and both serum and gastric mucosal MDA contents in the warm needling group and Omeprazole group were down-regulated significantly (P<0. 01), whereas both serum and mucosal SOD activity in the warm needling group and serum SOD activity in the Omeprazole group were up-regulated significantly (P<0. 01). Comparison between the warm needling group and Omeprazole group showed that the UI, both serum and gastric mucosal SOD activity, and serum MDA level of the former group were evidently higher than those of the Omeprazole group (P<0. 05), but gastric mucosal MDA level of the warm needling group was apparently lower than that of the Omeprazole group (P<0.05).

CONCLUSION

Warm-needle moxibustion can reduce the gastric lesion (ulcer) caused by aspirin in the rat, which may be closed associated with its effects in up-regulating both serum and gastric mucosal SOD activity and down-regulating both serum and gastric mucosal MDA levels.

摘要

目的

观察温针疗法对服用抗风湿剂量阿司匹林所致大鼠胃黏膜损伤的影响。

方法

将40只SD大鼠随机分为空白组、模型组、温针组和奥美拉唑（治疗消化性溃疡）组，每组10只。实验前大鼠禁食12小时、禁水4小时。空白组大鼠灌胃给予生理盐水（10 mL/kg）。通过灌胃给予阿司匹林（420 mg/kg）建立胃黏膜损伤模型。对“中脘”（CV 12）、“下脘”（CV 10）、“足三里”（ST 36）和“内关”（PC 6）进行温针治疗30分钟，每天1次，共7天。奥美拉唑组大鼠每天灌胃给予奥美拉唑（10 mg/100 g体重），共7天。处死大鼠后，按照Guth法计算胃黏膜溃疡指数（UI）。分别采用羟胺法和硫代巴比妥酸法检测血清及胃黏膜中超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平。

结果

与空白组比较，模型组大鼠胃UI、血清及胃黏膜MDA水平均明显升高（P<0.01），而模型组血清及胃黏膜SOD活性明显降低（P<0.01）。与模型组比较，温针组和奥美拉唑组大鼠胃UI、血清及胃黏膜MDA含量均显著下调（P<0.01），而温针组血清及胃黏膜SOD活性和奥美拉唑组血清SOD活性均显著上调（P<0.01）。温针组与奥美拉唑组比较，温针组大鼠UI、血清及胃黏膜SOD活性、血清MDA水平均明显高于奥美拉唑组（P<0.05），但温针组胃黏膜MDA水平明显低于奥美拉唑组（P<0.05）。