Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.
Biochemistry (Mosc). 2011 Apr;76(4):462-6. doi: 10.1134/s0006297911040092.
The activities of hydrolases (acid phosphatase, RNase, and proteases) in healthy and tobacco mosaic virus-infected leaves of Nicotiana tabacum L. var. Samsun, both untreated and treated with polysaccharides (PS) (1,3;1,6-β-D-glucan, fucoidan, and κ/β-carrageenan), were determined. The PS lead to substantial increase in the hydrolase level. The percentage of viral particles undergoing destructive change also increases in leaves treated with PS 24 h before infection. We suppose that the PS-mediated hydrolase activation promotes intracellular destruction of the viral particles and, thus, comprises one of the PS-induced protective mechanisms limiting intracellular viral accumulation.
研究了未感染和感染烟草花叶病毒的烟草（Nicotiana tabacum L. var. Samsun）叶片中水解酶（酸性磷酸酶、核糖核酸酶和蛋白酶）的活性，以及未经处理和用多糖（1,3；1,6-β-D-葡聚糖、褐藻聚糖胶和κ/β-卡拉胶）处理的叶片中的水解酶活性。多糖导致水解酶水平显著增加。在感染前 24 小时用多糖处理的叶片中，也会导致更多的病毒颗粒发生破坏性变化。我们假设，多糖介导的水解酶激活促进了病毒颗粒的细胞内破坏，因此，这是多糖诱导的保护机制之一，限制了细胞内病毒的积累。
