Dipartimento di Produzione Vegetale, Facoltà di Agraria, Università degli Studi di Milano, Milano, Italy.
J Pineal Res. 2011 Oct;51(3):278-85. doi: 10.1111/j.1600-079X.2011.00887.x. Epub 2011 May 17.
Resistance inducers are a class of agrochemicals, including benzothiadiazole and chitosan, which activate the plant own defence mechanisms. In this work, open-field treatments with plant activators were performed on two red grape (Vitis vinifera L.) varieties cultivated in different localities, Groppello (Brescia, Lombardia, Italy) and Merlot (Treviso, Veneto, Italy). Treatments were carried out every 10 days until the véraison and, after harvesting, experimental wines (microvinificates) were prepared. In general, both melatonin and total polyphenol content, determined by mass spectrometry and Folin-Ciocalteu assay, respectively, were higher in wines produced from grapes treated with resistance inducers than in those obtained from untreated control and conventional fungicide-treated grapes. Accordingly, antiradical power of wines derived from plant activator-treated grapes, measured by both DPPH (2,2-diphenyl-1-picrylhydrazyl) and the ABTS [(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical-scavenging assay, was higher than in their counterparts. To the best of our knowledge, this is the first report on the effects of agrochemicals on the melatonin content of red wine.
诱抗剂是一类农用化学品，包括噻苯隆和壳聚糖，它们能激活植物自身的防御机制。本研究在意大利伦巴第大区布雷西亚省种植的格罗佩罗（Groppello）和意大利威尼托大区特雷维索省种植的梅洛（Merlot）两种红葡萄品种上进行了大田诱抗剂处理。每隔 10 天处理一次，直至葡萄转色期，收获后，制备实验用葡萄酒（微型葡萄酒）。一般来说，用质量分析法和福林-肖卡法测定的葡萄酒中的褪黑素和总多酚含量，分别高于未用诱抗剂处理的对照和常规杀菌剂处理的葡萄所酿葡萄酒中的含量。相应地，用 DPPH（2,2-二苯基-1-苦肼基）和 ABTS[（2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）]自由基清除试验测定的来自用植物激活剂处理的葡萄的葡萄酒的抗自由基能力均高于其对应物。据我们所知，这是首次报道农用化学品对红葡萄酒中褪黑素含量的影响。
