Environmental and Ecological Modelling Group, UCD School of Mathematical Sciences, University College Dublin, Belfield, Dublin 4, Ireland, UCD School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
Mol Ecol Resour. 2008 Jan;8(1):95-8. doi: 10.1111/j.1471-8286.2007.01901.x.
ribosort is a computer package for convenient editing of automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (TRFLP) data. It is designed to eliminate the labourious task of manually classifying community fingerprints in microbial ecology studies. This program automatically assigns detected fragments and their respective relative abundances to appropriate ribotypes. It permits simultaneous sorting of multiple profiles and facilitates direct workflow from TRFLP and ARISA output through to community analyses. ribosort also provides several options to merge repeat profiles of a sample into a single composite profile. By creating a 'ribotypes by samples' matrix ready for statistical analyses, use of the package saves time and simplifies the preparation of DNA fingerprint data sets for statistical analysis. In addition, ribosort performs exploratory analysis on the data by creating multidimensional scaling plots that compare the similarity of sample profiles using the statistical software r.
ribosort 是一个方便编辑自动化核糖体基因间隔区分析 (ARISA) 和末端限制性片段长度多态性 (TRFLP) 数据的计算机软件包。它旨在消除微生物生态学研究中手动分类群落指纹图谱的繁琐任务。该程序可自动将检测到的片段及其各自的相对丰度分配给适当的核糖体型。它允许同时对多个图谱进行分类，并方便地直接从 TRFLP 和 ARISA 输出到群落分析。ribosort 还提供了几种选项，可将样本的重复图谱合并为单个复合图谱。通过创建一个准备进行统计分析的“按样本划分的核糖体型”矩阵，该软件包可节省时间，并简化 DNA 指纹图谱数据集的准备工作，以进行统计分析。此外，ribosort 通过使用统计软件 r 创建多维尺度图来对数据进行探索性分析，该图比较了使用统计软件 r 对样本图谱的相似性。
