School of Biological Sciences, Flinders University GPO Box 2100, Adelaide, SA 5001, Australia, Centre for Evolutionary Biology and Biodiversity, University of Adelaide, SA 5005, Australia.
Mol Ecol Resour. 2008 Jan;8(1):142-4. doi: 10.1111/j.1471-8286.2007.01900.x.
An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
采用富集技术分离了寄生蝇 Philornis downsi（双翅目：蝇科）的 11 个二、三、四微卫星。这些位点在单重或双重扩增体系中可用于 P. downsi 的 PCR 扩增。这些位点表现出低到中度多态性，具有 3-4 个等位基因，观察到的杂合度范围为 0.05-0.86。这些新标记将有助于进行种群水平和亲子分析，并为这种对脆弱鸟类物种具有高影响力的寄生虫的生态学提供有价值的信息。
