A. E. Wood Laboratory, Texas Parks & Wildlife Department, 507 Staples Road, San Marcos, TX 78666, USA, Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA.
Mol Ecol Resour. 2008 Jan;8(1):178-84. doi: 10.1111/j.1471-8286.2007.01917.x.
We isolated and characterized 52 novel microsatellite markers from Florida largemouth bass, Micropterus salmoides floridanus, for use in conservation, management and population genetic studies. Markers were assessed in M. s. floridanus from peninsular Florida (n = 30) and averaged eight alleles per locus with observed heterozygosity of 0.57 (range 0-0.97). Cross-taxa amplification was successful among 88% of tested congeners. These polymorphic and potentially taxon-diagnostic markers contribute to the limited number of microsatellites currently available for micropterids and specifically M. s. floridanus.
我们从佛罗里达鲈(Micropterus salmoides floridanus)中分离并鉴定了 52 个新的微卫星标记,用于保护、管理和种群遗传研究。在佛罗里达半岛的佛罗里达鲈(n=30)中评估了这些标记,每个位点平均有 8 个等位基因,观测杂合度为 0.57(范围为 0-0.97)。在测试的 88%近缘种中,交叉分类群扩增是成功的。这些多态性和潜在的分类群诊断标记增加了目前微卫星标记在微鳍鱼类中的数量,特别是在佛罗里达鲈中。