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开发和鉴定金刚鹦鹉微卫星标记。

Development and characterization of microsatellites markers from the macaw.

机构信息

Centro de Pesquisa e Desenvolvimento em Recursos Genéticos Vegetais - C P 28 Campinas, SP 13075-630, Brazil.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):224-6. doi: 10.1111/j.1471-8286.2007.01932.x.

DOI:10.1111/j.1471-8286.2007.01932.x
PMID:21585762
Abstract

Macaw (Acrocomia aculeata) is a native palm tree from tropical forests, highly abundant in Brazil and cited as one of the principal sources of plant oil, thus presenting a high potential for biodiesel production. We have optimized and utilized a set of eight polymorphic microsatellite markers for A. aculeata from an enriched genomic library. Automatic sequencing and fluorescence detection were employed to analyse 43 individuals from natural populations. In this study, we have obtained an average number of five alleles per locus. These loci will be employed in future studies of population genetics by providing subsidy information for the species conservation and genetic breeding.

摘要

金刚鹦鹉(Acrocomia aculeata)是一种原产于热带雨林的棕榈树,在巴西极为丰富,并被认为是植物油脂的主要来源之一,因此具有生产生物柴油的巨大潜力。我们从富集基因组文库中为 A. aculeata 优化并利用了一套 8 个多态性微卫星标记。自动测序和荧光检测用于分析 43 个来自自然种群的个体。在本研究中,每个位点平均获得了 5 个等位基因。这些标记将用于未来的种群遗传学研究,为物种保护和遗传育种提供辅助信息。

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