鉴定巴西热带草原药用植物大花紫玉盘（夹竹桃科）中的微卫星位点。
Characterization of microsatellite loci in Himatanthus drasticus (Apocynaceae), a medicinal plant from the Brazilian savanna.
机构信息
Instituto de Biologia, Departamento de Biologia Vegetal, Universidade Estadual de Campinas, CP 6109, Barão Geraldo, 13083-970 Campinas, São Paulo, Brazil.
出版信息
Am J Bot. 2011 Sep;98(9):e244-6. doi: 10.3732/ajb.1100135. Epub 2011 Aug 22.
PREMISE OF THE STUDY
We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. •
METHODS AND RESULTS
Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. •
CONCLUSIONS
The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species.
研究前提
我们开发了一套新的微卫星标记，用于研究巴西东北部民间医学中使用的 Janaguba 树（Himatanthus drasticus (Mart.) Plumel）的基因组。这些新标记正被用于评估对该物种自然种群遗传结构和多样性的收获影响。
方法和结果
从富集的 H. drasticus 基因组文库中分离出微卫星位点。九对引物成功扩增出多态性微卫星区域，每个位点平均有 8.5 个等位基因。观察到的和预期的杂合度平均值分别为 0.456 和 0.601。
结论
本文描述的微卫星标记是研究 H. drasticus 种群遗传学的有价值的工具。大多数引物也扩增了同一属中另一个物种的基因组序列。这组新的标记可能有助于设计该物种的遗传保护策略和可持续管理计划。
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