永久性遗传资源：康拉迪娜属（唇形科）微卫星位点的分离、鉴定和跨物种扩增。

PERMANENT GENETIC RESOURCES: Isolation, characterization and cross-species amplifications of microsatellite loci from Conradina (Lamiaceae).

机构信息

Department of Botany, University of Florida, Gainesville, FL 32611, USA, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):363-6. doi: 10.1111/j.1471-8286.2007.01957.x.

DOI:10.1111/j.1471-8286.2007.01957.x

PMID:21585793

Abstract

We report the isolation of microsatellite loci from three species in the genus Conradina (Lamiaceae). To ensure their utility for multiple species, loci were screened for amplification and variability in all six Conradina species; 11 loci demonstrated high levels of amplification and polymorphism in most species. These 11 loci were characterized in 20 individuals from one population of Conradina brevifolia; alleles per locus ranged from five to 15, and observed heterozygosity ranged from 0.30 to 0.90. These microsatellites will be used to clarify species limits, detect interspecific hybridization, and understand the partitioning of genetic variation in each species of Conradina.

摘要

我们报告了从唇形科 Conradina 属的三个物种中分离出的微卫星位点。为了确保它们在多个物种中的适用性，对所有六个 Conradina 物种进行了扩增和变异性筛选；11 个位点在大多数物种中表现出高水平的扩增和多态性。这 11 个位点在 Conradina brevifolia 的一个种群的 20 个个体中进行了表征；每个位点的等位基因数范围为 5 到 15，观察到的杂合度范围为 0.30 到 0.90。这些微卫星将用于阐明物种界限，检测种间杂交，并了解 Conradina 每个物种遗传变异的划分。