ESE, Bâtiment 360, Université Paris-Sud, 91405 Orsay, France, MIG-INRA Bâtiment 233, Domaine de Vilvert, 78350 Jouy en Josas Cedex, France, Department of Biology, Amherst College, Amherst, MA 01002, USA, Genoscope-CNRG CP 5706, 91057 Evry Cedex, France.
Mol Ecol Resour. 2008 Mar;8(2):387-92. doi: 10.1111/j.1471-8286.2007.01967.x.
We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M. dianthorum, collected, respectively, from the plants Silene latifolia, S. nutans, S. vulgaris and Dianthus carthusianorum. Intrapopulation polymorphism was investigated using 24 isolates, and cross-amplification was explored using 23 isolates belonging to at least 10 different Microbotryum species. This study provides numerous microsatellite markers for population genetics and mapping studies.
我们报告了在引起石竹科植物花药黑粉病的真菌复合体 Microbotryum 的四个物种上开发的 60 个微卫星标记。微卫星是在从分别采集自繁缕、长白剪股颖、普通瞿麦和香石竹的 M. lychnis-dioicae、M. violaceum sensus stricto、M. lagerheimii 和 M. dianthorum 分离物的四个表达序列标签（EST）文库中发现的。利用 24 个分离物研究了种群内多态性，并利用至少属于 10 个不同 Microbotryum 物种的 23 个分离物探索了交叉扩增。本研究为群体遗传学和作图研究提供了大量的微卫星标记。
