用于桉树茎溃疡病原菌 Kirramyces gauchensis 的微卫星标记。

Microsatellite markers for the Eucalyptus stem canker fungal pathogen Kirramyces gauchensis.

机构信息

Department of Genetics, Forestry and Agriculture Biotechnology Institute, University of Pretoria, 74 Lunnon Road, Hillcrest, Pretoria 0002, Gauteng, South Africa.

出版信息

Mol Ecol Resour. 2008 May;8(3):590-2. doi: 10.1111/j.1471-8286.2007.02005.x.

DOI:10.1111/j.1471-8286.2007.02005.x

PMID:21585841

Abstract

Ten microsatellite markers were developed for the fungus Kirramyces gauchensis, which causes an important stem canker disease of Eucalyptus trees in plantations. Primers for 21 microsatellite regions were designed from cloned fragments. Fourteen of the primer pairs provided single amplicons and 10 of these were polymorphic for K. gauchensis. Allelic diversity ranged from 0.21 to 0.76 with a total of 30 alleles. None of the markers was able to amplify in the phylogenetically distinct but morphologically similar species Kirramyces zuluensis. The 10 characterized polymorphic microsatellite regions will be studied to determine the population structure of K. gauchensis in plantations of different countries.

摘要

为引起桉树种植园重要茎溃疡病的真菌——糙皮侧耳（Kirramyces gauchensis）开发了 10 个微卫星标记。从克隆片段设计了 21 个微卫星区域的引物。其中 14 对引物提供了单扩增子，其中 10 对糙皮侧耳呈多态性。等位基因多样性范围从 0.21 到 0.76，共有 30 个等位基因。没有一个标记能够在形态相似但系统发育上不同的物种——津巴布韦侧耳（Kirramyces zuluensis）中扩增。将对这 10 个特征性多态微卫星区域进行研究，以确定不同国家种植园糙皮侧耳的种群结构。