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为高度濒危的巴氏蟾(Philoria frosti)分离多态性三核苷酸和四核苷酸微卫星位点。

Isolation of polymorphic tri- and tetranucleotide microsatellite loci for the highly endangered Baw Baw frog (Philoria frosti).

机构信息

Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia 5000, Australia, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, South Australia 5000, Australia, Department for Sustainability and Environment, 120 McCarthys Spur Road, Noojee, Victoria 3833, Australia.

出版信息

Mol Ecol Resour. 2008 May;8(3):593-5. doi: 10.1111/j.1471-8286.2007.02006.x.

Abstract

We isolated 15 polymorphic microsatellite loci for the Baw Baw frog, Philoria frosti, from a genomic library enriched for (AAC)(n) and (AAAG)(n) repetitive elements. The number of alleles ranges from two to 14 per locus with the observed heterozygosity ranging from 0.04 to 0.96.

摘要

我们从富含 (AAC)(n) 和 (AAAG)(n) 重复元件的基因组文库中分离出 15 个多态性微卫星位点用于巴氏蟾蛙,Philoria frosti。每个位点的等位基因数范围从 2 到 14 个,观察到的杂合度范围从 0.04 到 0.96。

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