Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Pretoria 0002, Gauteng, South Africa, African Coelacanth Ecosystem Programme, South African Institute for Aquatic Biodiversity, Private Bag 1015, Grahamstown 6140, South Africa.
Mol Ecol Resour. 2008 Sep;8(5):1065-7. doi: 10.1111/j.1755-0998.2008.02156.x. Epub 2008 Jun 28.
Eight polymorphic microsatellite loci, containing simple tetranucleotide repeats, were isolated de novo from a Pomatomus saltatrix partial genomic library using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. These loci were further characterized in 100 individuals from two putative populations off the South African east coast. The loci are highly polymorphic with 18-37 alleles (on average 24 alleles/locus) and the observed heterozygosity in both populations was high (0.79). These loci will be used to assess population structuring in P. saltatrix along the southern African coast with consideration of implications for future management of this important linefish species.
从南非东海岸两个假定群体的 100 个个体中，进一步对这 8 个微卫星位点进行了特征分析。这些微卫星位点是通过重复序列扩增片段长度多态性快速分离法，从尖海龙部分基因组文库中分离出来的，包含简单的四核苷酸重复。这些微卫星位点高度多态，每个位点有 18-37 个等位基因（平均每个位点 24 个等位基因），两个群体的观察杂合度均较高（0.79）。这些微卫星位点将用于评估南非南部沿海尖海龙的种群结构，并考虑对这一重要的鱼类物种进行未来的管理。
