高度多态性微卫星位点的大加那利岛石龙子（Chalcides sexlineatus）及其在其他加那利群岛 Chalcides 中的适用性。

Highly polymorphic microsatellite loci for the Gran Canarian skink (Chalcides sexlineatus) and their applicability in other Canarian Chalcides.

机构信息

Departamento de Genética, Facultad de Medicina, Universidad de Las Palmas de Gran Canaria, Las Palmas, 35080, Gran Canaria, Spain, School of Biological and Earth Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK.

出版信息

Mol Ecol Resour. 2008 May;8(3):666-8. doi: 10.1111/j.1471-8286.2007.02040.x.

DOI:10.1111/j.1471-8286.2007.02040.x

PMID:21585865

Abstract

The scincid lizard Chalcides sexlineatus is endemic to the island of Gran Canaria, within which it shows a pronounced phylogeographical pattern. Understanding the maintenance of this pattern requires knowledge of nuclear DNA structuring. Eleven polymorphic tetranucleotide microsatellite loci were isolated from a library constructed from genomic DNA enriched for AAAG repeat sequence. The number of alleles per locus ranged from 12 to 19, and expected heterozygosities spanned from 0.738 to 0.933 (n = 30). The majority of these could be cross-amplified in other endemic Canarian Chalcides. The loci will be used to investigate gene flow between the principal mitochondrial clades within C. sexlineatus.

摘要

细纹石龙子 Chalcides sexlineatus 是加那利群岛特有的物种，在该群岛内表现出明显的系统地理格局。了解这种格局的维持需要了解核 DNA 结构。从富含 AAAG 重复序列的基因组 DNA 文库中分离出 11 个多态性四核苷酸微卫星基因座。每个基因座的等位基因数范围为 12 到 19，预期杂合度从 0.738 到 0.933（n=30）。这些基因座中的大多数可以在其他加那利群岛特有的 Chalcides 中交叉扩增。这些基因座将用于研究 C. sexlineatus 内主要的线粒体类群之间的基因流。