Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
Mol Ecol Resour. 2008 Jul;8(4):796-8. doi: 10.1111/j.1755-0998.2007.02067.x.
Eleven microsatellites were isolated from a genomic DNA library enriched for CA- and GA-repeats, and were characterized in 48 individuals of red swamp crayfish Procambarus clarkii. All 11 microsatellites were polymorphic with an average of allele number of 6.6 per locus. Genotypic distributions of these 11 markers were found to be in conformance with the expectations of Hardy-Weinberg equilibrium. All 11 markers were unlinked. These markers are being used to study the invasion routine, genetic diversity and population structure of the species P. clarkii.
从富含 CA 和 GA 重复序列的基因组 DNA 文库中分离出 11 个微卫星，并用 48 只红沼泽螯虾 Procambarus clarkii 个体对其进行了特征描述。这 11 个微卫星均具有多态性，每个位点的平均等位基因数为 6.6。这些标记的基因型分布符合哈迪-温伯格平衡的预期。这 11 个标记均不连锁。这些标记正在用于研究物种 P. clarkii 的入侵规律、遗传多样性和种群结构。
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