永久性遗传资源：100 个红色鼓鱼（Sciaenops ocellatus）微卫星的 PCR 引物。

PERMANENT GENETIC RESOURCES: PCR primers for 100 microsatellites in red drum (Sciaenops ocellatus).

机构信息

Center for Biosystematics and Biodiversity, Texas A & M University, TAMU-2258, College Station, TX 77843-2258, USA, National Center for Cool and Cold Water Aquaculture, Kearneysville, WV 25430, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):393-8. doi: 10.1111/j.1471-8286.2007.01969.x.

DOI:10.1111/j.1471-8286.2007.01969.x

PMID:21585801

Abstract

One hundred nuclear-encoded microsatellites from a genomic library of red drum (Sciaenops ocellatus) were isolated and characterized. Eight microsatellites had tetranucleotide motifs; 92 had dinucleotide motifs. The average number of alleles per microsatellite (sample of 22-24 fish) was 17.7 (range = 2-30); gene diversity averaged 0.796 (range = 0.227-1.000). Following Bonferroni correction, genotype frequencies at 90 microsatellites did not deviate significantly from Hardy-Weinberg equilibrium expectations. Occurrence of null alleles was inferred at 15 microsatellites; alleles differing by only a single base were observed at 11 microsatellites. The microsatellites developed should prove useful for population-genetic studies of 'wild' red drum and in construction of a genetic map.

摘要

从红鼓鱼（Sciaenops ocellatus）的基因组文库中分离和鉴定了 100 个核编码微卫星。8 个微卫星具有四核苷酸基序；92 个具有二核苷酸基序。每个微卫星（22-24 条鱼样本）的平均等位基因数为 17.7（范围为 2-30）；基因多样性平均为 0.796（范围为 0.227-1.000）。经过 Bonferroni 校正后，90 个微卫星的基因型频率与 Hardy-Weinberg 平衡预期没有显著偏离。在 15 个微卫星中推断出存在无效等位基因；在 11 个微卫星中观察到仅相差一个碱基的等位基因。开发的微卫星应该对“野生”红鼓鱼的种群遗传研究以及遗传图谱的构建有用。