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为 Neotropical 药用树木 Casearia sylvestris Sw.(杨柳科)分离和鉴定微卫星标记。

Isolation and characterization of microsatellite markers for Casearia sylvestris Sw. (Salicaceae), a neotropical medicinal tree.

机构信息

UNESP-Universidade Estadual Paulista 'Julio de Mesquita Filho', Departamento de Genética, Distrito de Rubião Junior s/n, Botucatu 18618-000, SP, Brazil.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):802-4. doi: 10.1111/j.1755-0998.2007.02069.x.

Abstract

Casearia sylvestris Sw. is a widespread neotropical tree utilized in popular medicine. Recent research ranked Casearia as one of the most promising genus in the search of drugs against cancer. Despite its wide distribution and pharmacological importance, no microsatellite markers have yet been developed for this genus. In this study, we provide 10 polymorphic microsatellite loci specifically designed for C. sylvestris, used to analyse 90 individuals distributed in two populations from São Paulo state, Brazil. On average, 12.3 alleles per locus were identified, showing the ability of the markers to detect microsatellite polymorphism in this species.

摘要

山黄麻(Casearia sylvestris Sw.)是一种广泛分布于新热带地区的乔木,被用于传统医学。最近的研究将山黄麻列为抗癌药物研发最有前景的属之一。尽管山黄麻分布广泛且具有药理学重要性,但尚未为该属开发出微卫星标记。本研究提供了 10 个专门为山黄麻设计的多态性微卫星标记,用于分析来自巴西圣保罗州的两个种群的 90 个个体。平均每个位点检测到 12.3 个等位基因,表明这些标记能够检测该物种中的微卫星多态性。

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