Bueno Paula Carolina Pires, Pereira Fabiola Manhas Verbi, Torres Roseli Buzanelli, Cavalheiro Alberto José
Institute of Chemistry, Department of Organic Chemistry, São Paulo State University - UNESP, Araraquara/SP, Brazil.
Institute of Chemistry, Department of Analytical Chemistry, São Paulo State University - UNESP, Araraquara/SP, Brazil.
J Sep Sci. 2015 May;38(10):1649-56. doi: 10.1002/jssc.201401421. Epub 2015 Apr 20.
This work describes the development and optimization of an analytical method utilizing liquid chromatography and chemometrics to evaluate and differentiate two varieties of Casearia sylvestris Swartz (Salicaceae) from São Paulo State (Brazil) based on their secondary metabolite profiles. Previously, analytical studies only concerned the analysis of clerodane-type diterpenes. Therefore, considering the importance of including phenolic compounds in such analysis, we used design of experiments to simultaneously extract and detect the largest number of compounds from both chemical classes. This new strategy allowed a comprehensive chromatographic analysis of C. sylvestris, and the results for the two varieties exhibited an interesting distribution according to their original ecosystems, suggesting a strong correlation to the main metabolites found in each species group. Besides their inherent morphological differences, C. sylvestris variety lingua, mainly found in Cerrado areas, predominantly contains phenolic compounds, while C. sylvestris variety sylvestris, mainly found in Atlantic Forest areas, contains mostly clerodane-type diterpenes. Finally, it was also possible to observe differences in the secondary metabolite composition within each group depending on the place where samples were collected.
这项工作描述了一种分析方法的开发与优化，该方法利用液相色谱法和化学计量学，基于其次级代谢产物谱来评估和区分来自巴西圣保罗州的两种南美桤叶树（杨柳科）。此前，分析研究仅关注克罗烷型二萜类化合物的分析。因此，考虑到在此类分析中纳入酚类化合物的重要性，我们采用实验设计来同时从这两类化学物质中提取和检测数量最多的化合物。这种新策略实现了对南美桤叶树的全面色谱分析，两个变种的结果根据其原始生态系统呈现出有趣的分布，表明与每个物种组中发现的主要代谢产物有很强的相关性。除了其固有的形态差异外，主要生长在塞拉多地区的南美桤叶树变种lingua主要含有酚类化合物，而主要生长在大西洋森林地区的南美桤叶树变种sylvestris主要含有克罗烷型二萜类化合物。最后，还可以观察到每组内次级代谢产物组成因样品采集地点的不同而存在差异。
