Department of Fish and Wildlife Resources, University of Idaho, Moscow, ID 83844-1136, USA.
Mol Ecol Resour. 2008 Jul;8(4):835-9. doi: 10.1111/j.1755-0998.2007.02083.x.
Short amplicon primers were redesigned for 17 microsatellite loci developed in St. Vincent's Amazon and six loci developed in blue-and-yellow macaw and tested using six species of Neotropical parrot. Polymorphism was observed at 12 loci in blue-and-yellow macaw, 10 in red-and-green macaw, 11 in scarlet macaw, 10 in chestnut-fronted macaw, 11 in red-bellied macaw and 16 in mealy parrot. Number of alleles per locus ranged from two to 23 and expected heterozygosity ranged from 0.05 to 0.95. The resulting multiplexed loci will be useful in evaluating genetic diversity, genetic structure and mating system in Neotropical parrots.
短扩增子引物被重新设计用于圣文森特亚马逊地区开发的 17 个微卫星位点和在蓝黄金刚鹦鹉中开发的 6 个位点，并使用六种新热带鹦鹉进行了测试。在蓝黄金刚鹦鹉中观察到 12 个位点的多态性，在红绿金刚鹦鹉中观察到 10 个，在猩红金刚鹦鹉中观察到 11 个，在栗额金刚鹦鹉中观察到 10 个，在红腹金刚鹦鹉中观察到 11 个，在和尚鹦鹉中观察到 16 个。每个位点的等位基因数从 2 到 23 不等，预期杂合度从 0.05 到 0.95 不等。由此产生的多重位点将有助于评估新热带鹦鹉的遗传多样性、遗传结构和交配系统。
