Wildlife Research Center of Kyoto University, 2-24 Tanaka-Sekiden-cho, Sakyo-ku, Kyoto, 606-8203, Japan.
Mol Biol Rep. 2012 Jan;39(1):693-9. doi: 10.1007/s11033-011-0787-1. Epub 2011 May 28.
Using mitochondrial and nuclear markers species identification was conducted in the case of seized feathers. Earlier, we had sequenced cytochrome c oxidase subunit I (COI) both from 10 seized specimens and 43 validation specimens from captive macaws belonging to 4 Ara species (A. macao, A. chloropterus, A. ararauna, and A. ambiguus) and identified 19 haplotypes based on COI sequences. Species-level identification using Barcode of Life Data Systems showed that seized feathers shared the highest similarity with scarlet macaws (A. macao), and this result was supported by the tree-base identification with high bootstrap values. Moreover, microsatellite profiles in AgGT17 locus showed that patterns of allelic distribution in the seized feathers were apparently distinct from those of red-and-green macaw (A. chloropterus), but were overlapped with those of A. macao, suggesting that all of seized feathers were derived from several individuals of A. macao. We also determined the parentage of hybrid macaws by the combination of COI barcodes and microsatellite profiles. The technique presented here will contribute to forensic identification and future conservation of large macaws that have been lost due to deforestation.
使用线粒体和核标记进行物种鉴定，涉及被查获的羽毛。此前，我们已经对 10 个被查获的标本和 43 个来自人工饲养金刚鹦鹉的验证标本进行了细胞色素 c 氧化酶亚基 I（COI）的测序，这些标本属于 4 个 Ara 物种（A. macao、A. chloropterus、A. ararauna 和 A. ambiguus），并根据 COI 序列确定了 19 个单倍型。基于生命条形码数据系统的物种鉴定显示，被查获的羽毛与猩红金刚鹦鹉（A. macao）的相似度最高，这一结果得到了基于高自举值的树基鉴定的支持。此外，AgGT17 基因座的微卫星图谱显示，被查获的羽毛的等位基因分布模式明显不同于红绿金刚鹦鹉（A. chloropterus），但与 A. macao 重叠，表明所有被查获的羽毛都来自几个 A. macao 个体。我们还通过 COI 条形码和微卫星图谱的结合来确定杂交金刚鹦鹉的亲子关系。本研究提供的技术将有助于对因森林砍伐而失去的大型金刚鹦鹉进行法医鉴定和未来保护。
