School of Biological Sciences, Washington State University, Pullman, WA 99164, USA.
Mol Ecol Resour. 2008 Jul;8(4):936-8. doi: 10.1111/j.1755-0998.2008.02123.x.
Thirteen polymorphic microsatellite loci were identified and developed for the coastal tailed frog, Ascaphus truei, from sites within the Olympic Peninsula of Washington, USA. These tetranucleotide repeat loci were highly variable, averaging 19 alleles per locus and expected heterozygosity of 0.91. In addition, these loci cross-amplify in the sister species, Ascaphus montanus. These markers will prove useful in identifying fine-scale genetic structure, as well as provide insight into the evolution and conservation of this group across fragmented landscapes.
本研究在美国华盛顿奥林匹克半岛的沿海蟾属（Ascaphus truei）的多个地点，鉴定和开发了 13 个多态性微卫星位点。这些四核苷酸重复位点具有高度的变异性，平均每个位点有 19 个等位基因，预期杂合度为 0.91。此外，这些位点在亲缘种，高山蟾属（Ascaphus montanus）中可以扩增。这些标记将有助于鉴定细微的遗传结构，并深入了解该群体在破碎化景观中的进化和保护。
