Unidad de Genética Forestal, Instituto Nacional de Tecnología Agropecuaria, EEA Bariloche, CC277, 8400 Bariloche, Argentina, Istituto di Genetica Vegetale, CNR, 50019 Sesto Fiorentino (Firenze), Italy, Dipartimento di Biotecnologie Agrarie, Laboratorio Genexpress, 50019 Sesto Fiorentino (Firenze), Italy, Departamento Sistemas y Recursos Forestales, CIFOR-INIA, 28040 Madrid, Spain.
Mol Ecol Resour. 2008 Jul;8(4):887-9. doi: 10.1111/j.1755-0998.2008.02099.x.
An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Numbers of alleles ranged from 3 to 19 and levels of observed heterozygosity among the 9 loci ranged from 0.32 to 0.95. No locus combinations exhibited linkage disequilibrium. These polymorphic markers will be useful tools for the study of demography and gene flow and more in general for population and conservation genetics of this species.
构建了一个丰富的基因组文库，并为安第斯巴塔哥尼亚地区最重要的经济树种南洋杉开发了 9 个新的多态性四核苷酸微卫星标记。在来自两个种群的 48 个个体中研究了这些标记的多态性。等位基因数范围为 3 至 19，9 个位点的观察杂合度水平范围为 0.32 至 0.95。没有发现任何位点组合存在连锁不平衡。这些多态性标记将是研究该物种的种群动态和基因流以及更普遍的种群和保护遗传学的有用工具。
