Freshwater Fisheries Research Center, Glami Lemi, 71650 Titi, Jelebu, Malaysia, Biology Department, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia.
Mol Ecol Resour. 2008 Sep;8(5):983-5. doi: 10.1111/j.1755-0998.2008.02127.x. Epub 2008 Jun 28.
Eight single locus microsatellite markers were developed to characterize the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. These microsatellites were isolated from an enriched genomic library contained by using a 5'-anchored polymerase chain reaction technique. Primers were designed to flank the repeat sequences and subsequently used to characterize 30 unrelated individuals of the giant freshwater prawn. The polymerase chain reaction amplification products of these eight microsatellite loci were polymorphic with the number of alleles ranging from two to 10 alleles per locus while the levels of heterozygosity ranged from 0.6333 to 0.8667.
从富集基因组文库中利用 5’端锚定聚合酶链反应技术分离出 8 个单基因座微卫星标记，用于鉴定马来西亚巨型淡水虾Macrobrachium rosenbergii。设计引物以侧翼重复序列，随后用于鉴定 30 个巨型淡水虾无关个体。8 个微卫星位点的聚合酶链反应扩增产物具有多态性，每个位点的等位基因数从 2 到 10 个不等，杂合度水平从 0.6333 到 0.8667。
