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Isolation of trinucleotide microsatellite markers for Mystus nemurus.

作者信息

Hoh B P, Siraj S S, Tan S G, Yusoff K

机构信息

Genetic Laboratory, Department of Biology, Faculty of Science, University Putra Malaysia.

出版信息

Genetika. 2008 Mar;44(3):427-9.

PMID:18664151
Abstract

Twelve single locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in South East Asia. They were obtained by using a rapid method namely the 5' anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291).

摘要

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