Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575 002, India.
Mol Ecol Resour. 2008 Sep;8(5):1040-2. doi: 10.1111/j.1755-0998.2008.02148.x. Epub 2008 Jun 28.
Eight microsatellite loci were identified and characterized in the commercially important giant freshwater prawn, Macrobrachium rosenbergii. The microsatellite loci were detected by the random screening for dinucleotide repeat units within a partial genomic library developed for the species with biotinylated probes (CA)(15) , (AT)(15) and (GA)(15) . All the eight loci were found to be polymorphic. The number of alleles and observed heterozygosities per locus ranged between three to 16 and 0.22 to 0.71, respectively. These microsatellite markers will be useful for the conservation and management of wild and cultured stocks and population genetic studies of freshwater prawn.
鉴定并描述了 8 个微卫星位点，这些位点存在于重要的商业性淡水虾品种罗氏沼虾中。通过生物素标记探针（CA）（15）、（AT）（15）和（GA）（15）对物种部分基因组文库中双核苷酸重复单元的随机筛选，检测到了这些微卫星位点。所有 8 个位点均表现出多态性。每个位点的等位基因数和观测杂合度分别在 3 到 16 个和 0.22 到 0.71 之间。这些微卫星标记将有助于对野生和养殖种群的保护和管理以及对淡水虾的种群遗传研究。
