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开发八种多态微卫星用于 Zn/Cd 超富集植物Sedum alfredii Hance(景天科)。

Development of eight polymorphic microsatellites for a Zn/Cd hyperaccumulator Sedum alfredii Hance (Crassulaceae).

机构信息

South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou 510650, China, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China, Graduate School of the Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):1049-51. doi: 10.1111/j.1755-0998.2008.02151.x. Epub 2008 Jun 28.

DOI:10.1111/j.1755-0998.2008.02151.x
PMID:21585968
Abstract

Sedum alfredii is a Zn/Cd hyperaccumulator distributed in East Asia. A total of eight polymorphic microsatellite markers were developed. These loci were screened in 25 individuals from one heavy metal-tolerant population and one nontolerant population, respectively. The average allele number of these markers was 5.25 per locus, ranging from two to nine. Population-specific alleles were found at each locus. The observed and expected heterozygosities ranged from 0.000 to 0.640 and from 0.451 to 0.819. Significant deviation from Hardy-Weinberg equilibrium was detected at both the species and the population level. No significant linkage disequilibrium was detected at population level.

摘要

阿尔泰景天是一种分布在东亚的 Zn/Cd 超积累植物。本研究共开发了 8 个微卫星标记,分别对来自一个耐重金属种群和一个非耐重金属种群的 25 个个体进行了筛选。这些标记的平均等位基因数为每个位点 5.25,范围为 2 到 9。每个位点都发现了种群特异性等位基因。观察到的和预期的杂合度范围分别为 0.000 到 0.640 和 0.451 到 0.819。在物种和种群水平都检测到了与 Hardy-Weinberg 平衡的显著偏离。在种群水平没有检测到显著的连锁不平衡。

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