半夏微卫星标记的分离与鉴定及跨物种扩增。

Isolation and characterization of microsatellite markers for Pinellia ternata and cross-species amplification.

机构信息

Institute of Molecular Ecology, MOE Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1460-2. doi: 10.1111/j.1755-0998.2008.02326.x. Epub 2008 Sep 15.

DOI:10.1111/j.1755-0998.2008.02326.x

PMID:21586076

Abstract

In this study, we isolated and characterized 12 microsatellite loci for Pinellia ternata. Polymorphism of these 12 loci was assessed in 46 individuals collected from two wild populations. All the loci were polymorphic with four to 13 alleles per locus and the observed and expected heterozygosities ranged from 0.312 to 0.680 and from 0.506 to 0.734, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium (P < 0.05). No significant linkage disequilibrium was observed between pairs of studied loci. In addition, most markers amplified successfully in three closely related taxa that are Pinellia cordata, P. peltata and P. pedatisecta. These microsatellite markers could provide a useful tool for genetic structure studies of the Pinellia species.

摘要

在这项研究中，我们从两个野生种群中分离并鉴定了 12 个半夏微卫星位点。对 46 个个体的 12 个位点的多态性进行了评估。所有位点均具有多态性，每个位点有 4 到 13 个等位基因，观察到的和预期的杂合度分别为 0.312 到 0.680 和 0.506 到 0.734。没有一个位点偏离哈迪-温伯格平衡（P < 0.05）。研究的位点之间没有观察到显著的连锁不平衡。此外，大多数标记在三个密切相关的种中成功扩增，即三叶半夏、掌叶半夏和球茎半夏。这些微卫星标记可以为半夏属物种的遗传结构研究提供有用的工具。